Custom lentiviral vectors

Start: lentiviral vectors for immortalized cell lines

Benefits

• Very high level of transduction on permissive and “hard-to-transfect” cell lines
• No antibiotic selection required

Vectalys Start is dedicated to the transduction of immortalized cell lines. Vectalys Start allows for the transduction of permissive cell lines but also immortalized cell lines which are deemed to be very difficult to transfect.

Discover all immortalized cells transduced with 100% efficiency using Vectalys start lentiviral batch.

Features

• Titre (TU/ml) : Number of lentiviral functional particles leading to integration of vector DNA into the host genome.
• PP/TU ratio : Gives a measure of the lentiviral batch quality by discriminating the non functional physical particles (PP) from the functional particles.
• Specific activity (TU/mg) : Gives the ratio between functional particles and contaminating proteins in the batch. Specific activity (SA) increases with the purity of the batch.

Premium: lentiviral vectors for primary and stem cells

Benefits

• Very high level of transduction on primary cells
• No antibiotic selection required
• No induced toxicity or cell damages thanks to the low impurity content

Vectalys Premium is dedicated to the transduction of primary cells and stem cells for in vitro, in vivo or ex vivo experiments. Vectalys Premium’ high purity and titer eliminate the deleterious phenotypic changes that typically occur following transduction of target cells (such as loss of a sub-population, effects on proliferation, viability and differentiation of transduced cells).

Discover all primary and stem cells transduced with almost 100% efficiency using Vectalys premium lentiviral batch.

Features

• Titre (TU/ml) : Number of lentiviral functional particles leading to integration of vector DNA into the host genome.
• PP/TU ratio : Gives a measure of the lentiviral batch quality by discriminating the non functional physical particles (PP) from the functional particles.
• Specific activity (TU/mg) : Gives the ratio between functional particles and contaminating proteins in the batch. Specific activity (SA) increases with the purity of the batch.

Such batches are provided from 1ml to 25ml. Find out more about large scale productions of lentiviral vectors here.

Expert: lentiviral vectors for in vivo experiments

Benefits

• Get your experimental disease model in only one efficient transduction.
• Safe and clinically suitable delivery of transgenes without inducing toxicity or immunogenicity
• Gene transfer into adult animals for inflammatory or vaccine approaches

Vectalys Expert is the highest quality of lentiviral vectors on the market. It is dedicated to specific in vivo injections such as oocytes transduction, vaccine or inflammatory animal studies, direct tissue injection for in vivo gene therapy, transgenesis or direct tissue experiments, all requiring a high level of purity. This purification level shows minimal toxicity and immunogenicity after transduction.

Features

• Titre (TU/ml) : Number of lentiviral functional particles leading to integration of vector DNA into the host genome.
• PP/TU ratio : Gives a measure of the lentiviral batch quality by discriminating the non functional physical particles (PP) from the functional particles.
• Specific activity (TU/mg) : Gives the ratio between functional particles and contaminating proteins in the batch. Specific activity (SA) increases with the purity of the batch.

Your lentiviral vectors are fully customizable

Vectalys offers many possibilities for the production of lentiviral vectors carrying your gene of interest:

• Choose your promoter of choice among constitutive (EF1a, CMV) or inducible (TRE3G) , ubiquitous or specific (tissue-specific, cell-state specific, differentiation pathway specific, …) , strong or weak.
• Any sequence of interest can be carried: cDNA, shRNA, shMIR, sgRNA, multicistron (2A peptides, IRES….).
• Select reporters as needed: fluorescent proteins ( click here to see fluorescent proteins available and their physical properties), bioluminescent protein, antibiotic resistance, tag….
• Limit of 10kb for the encapsidation of the full cassette of interest.