What our experts have to say

AAV vs iLV
Let’s say you have one or several great gene candidates, you are done with all the in vitro studies and you have been asked to validate your results in vivo. Looking at the bibliography, you inevitably fall into thousands of data showing wonderful results using the Adenoviral Associated Vectors (AAVs). Now you should be asking yourself “what serotype do I need to choose for my project?” and there you go back to bibliography trying to find out a clear answer to this question… and you won’t.

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- Electroporation,
- Lipid or chemical transfection,
- Viral vectors…

A wide range of tools is available for gene transfer experiments. Among these, the lentiviral vector technology has proven to be an effective tool with significant benefits. This is mainly due to its efficiency, its gentleness with delicate cells, the stability of expression and the shortened time lapse of experiments. Unfortunately, results are not always living up to expectations. To prevent failure, several parameters must be taken into account. We describe in this article 10 best practices to follow for a good transduction efficiency.

Electroporation vs LentiFlash
Delivery, delivery, delivery!

Dr Jennifer Doudna opened the last American Society of Gene and Cell Therapy Annual Meeting in Washington by highlighting the current challenges of gene editing :
• Delivery
• Controlling repair pathways
• Ethics