Why not generate your own fluorescent reporter cell line?
Vectalys provides lentiviral vectors with constitutive fluorescent reporter to do that easily. With a single batch (5 fold cheaper than a cell line), you can generate several types of fluorescent cell lines.
You never have done this kind of experiment? Do not worry, our experts have created a simple guide with three steps to generate your own reporter cell lines with success. For more details, download the transduction protocol below:
Generation of reporter cell line: proof of concept
To give you an example, our products have already been used for reporter cell line generation and in vivo injections as described below:
Figure 1: generation of reporter cell line (prostatic PC3 cell line) using Vectalys' lentiviral particles and in vivo injection
Figure 2: tumor growth and metastasis visualization using luminescent and fluorescent cell lines
NB: for the development of a stable tumor cell line, it is possible to make a clonal selection of the parental generated cell line by limiting dilution. This gives the possibility to select the levels of expression in fluorescence and luminescence adapted to the requirements (subcutaneous grafts, orthotopic grafts etc.).
Lentiviral vectors vs reporter cell lines: benefits and drawbacks.
*The use of lentiviral derived vectors or genetically modified cells requires laboratory biosafety procedures and practices in accordance with your country regulations:
If you have any additional question related to the transduction protocol, please feel free to contact our technical support at firstname.lastname@example.org.