Vectalys offers a wealth of expertise in viral gene transfer technology for immortalized, primary and stem cells. The multiple benefits of viral transduction include the establishment of stable cell lines in a very short time without the use of antibiotics and the control of integrated copy number regardless of cell types and species. Vectalys offers a large range of customized services for an optimal and fast cell model generation and cell engineering of any cell types.
Stable cell lines with lentiviral vectors
Lentiviral vectors help resolve the issues with transfection techniques for the generation of stable cell lines.
The use of lentiviral vectors ensures high gene transfer efficiency and a stable expression when compared to conventional transfection techniques. Most transfection issues are related to gene transfer efficiency into hard-to-transfect cells, like lymphoid or myeloid cell lines, as well as primary or stem cells.
When using lentiviral vectors, efficiency issues no longer exist and the expression of the sequence of interest (cDNA, shRNA, miRNA) is stable in a single transduction, thanks to DNA integration into the host genome. In obtaining stable cell lines, lentiviral vectors save time, money and energy.
Cell engineering with lentiviral vectors
In delicate cells such as hematopoietic lineages for example, classical transfection protocols are not only transient and inefficient, they also induce cytotoxicity effects and proliferative arrests. Gene transfer using highly concentrated and purified lentiviral vectors allows for efficient and safe transduction of such cells.
Lentiviral vectors : a tool of choice for cell engineering
Lentiviral vectors are the engineering tool of choice to study and engineer primary cells without interfering with the cell phenotype, and are also the only tool capable of transducing primary cells without toxicity.
Additionally, the advantages of lentiviral vectors combined with our new technology LentiFlash allow for a faster expression without genomic traces in the target primary cells.
In vivo models with lentiviral vectors
In vivo, lentiviral vectors are efficient tools for noninvasive analysis of tumor animal models. They can be used through direct injection into adult animals or through in vitro transductions of any tumoral cells subsequently reimplanted in the context of cancer models.
Lentiviral vectors have to be highly concentrated and purified in order to achieve an effective transgene expression while maintaining the cells original phenotype, proliferation and viability. These criteria for the development of trustworthy and predictive cancer models are fulfilled by the properties of our lentiviral vectors, building a bridge between in vitro assays, in vivo results and finally therapeutic applications.