LentiFlash®: an RNA delivery lentiviral particle
LentiFlash®, a non-integrative lentiviral particle dedicated to cell and gene therapy applications, allows:
- A safe and efficient non-viral RNA delivery
- A short-term expression of your sequence of interest without retro-transcription nor integration
- A transient RNA expression without genomic trace
A safe and efficient RNA delivery into any cell type
LentiFlash® particles are VSV-G pseudotyped and highly purified. These two criteria make LentiFlash® very efficient for the transfer of biologically active RNAs into various cell types including immortalized, primary and stem cells.
As shown figure 1, LentiFlash® can transduce at a high level human primary T cells, a clinically target which is not easily transfected using electroporation or chemical transfection methods.
Figure 1: Transduction efficiency of human T cells using LentiFlash® carrying ZsGreen RNA compared to Vectalys' integrative lentiviral vectors.
Additionally, LentiFlash® does not require any selection process and preserves the original cell phenotype, its proliferation ability and a full viability.
A transient and short-term expression:
LentiFlash® delivers mRNA directly into the cytoplasm leading to a high, fast (4h post-transduction) and short-term expression of your sequence of interest. These assets make LentiFlash® particularly suitable to carry CRISPR-Cas9 into any cell type while preserving original cell phenotype and viability. RNA delivered by LentiFlash® technology is rapidly bioavailable. (Figure 2)
Figure 2: in vitro and in vivo data with LentiFlash® particles carrying luciferase RNA
Figure 3: Comparison of the luciferase expression with several gene delivery methods: pLuc (plasmid carrying luciferase DNA), LentiFlash® carrying Luciferase RNA, IDLV (integrase deficient lentiviral vectors carrying Luciferase DNA) and ILV-Luc (Lentiviral vectors carrying Luciferase DNA)
LentiFlash® allows a faster expression than ILV (integrative lentiviral vectors), IDLV (integrase deficient lentiviral vectors) and transfection technologies (pLuc) without genomic traces.
To see more data, please click on the following link Nature publishing group.
An RNA-delivery system based on a bacteriophage coat protein
LentiFlash® is an RNA delivery system based on a bacteriophage-lentivirus chimera. It has been constructed by exploiting a bacteriophage coat protein and its cognate 19-nt stem loop instead of the natural lentiviral Psi packaging sequence to achieve mRNA packaging into the lentiviral vectors.
Figure 4: LentiFlash production process
Applications of LentiFlash® technology
Thanks to the high and short-term expression of the transferred RNA LentiFlash® is particularly suitable for: