Vectalys’ know how

Vectalys provides highly purified and super concentrated lentiviral vectors that efficiently transfer genes into any cell type without affecting the original cell phenotype and viability.

Expertise in lentiviral vector production since 2005

Using its own proprietary technology, Vectalys has developed three production and purification processes to provide the right lentiviral vector for the right application. Since the creation of the company in 2005, we have generated more than 6000 lentiviral vectors batches for many customer applications.

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A unique patented purification process

Vectalys differs from all other lentiviral vectors producers by its purification offers adapted to client projects.

Using classical production processes, the proteins impurities co-purified with lentiviral vectors are responsible for toxicity observed in target cells after transduction.

Through a patented process involving serum and antibiotic free media and followed by several purification steps, Vectalys obtains the removal of 99% of proteins and DNA impurities. Vectalys provides the highest vector purity on the market.

These purification steps avoid phenotype changes or specific cell responses. The resulting high quality of lentiviral vectors allows to efficiently transduce delicate cells or hard to transfect cells.

A robust and strong titration method

The lentiviral vectors titer in TU/ml we provide represents the true count of efficient particles that our customers can use for their experiments. Most of our competitors do not provide titers in TU/ml units but the full count of particles, including damaged or empty particles which bring nothing but toxicity to the target cells.

During the production of viral vectors, functional and non functional particles are produced. A functional and complete particle is called a transduction unit (TU). Physical Particles (PP) represent functional particles and also non functional particles including empty particles, damaged particles and free p24 proteins. The PP/TU ratio is a good measure of the quality of the vector produced.

To increase the ratio of functional particles, it is essential to reduce the production of PP in the viral supernatants. This is another advantage of having a reliable and discriminating titration method for the different types of particles.

Integrative and non-integrative lentiviral technologies

Depending on your application, Vectalys provides both integrative lentiviral vectors and non-integrative LentiFlash particles.

Current lentiviral vectors, which deliver DNA integrated into the genome, are a leading delivery method for certain applications such as gene addition, stable cell line generation or cancer immunotherapy, which require a stable and long-term gene expression.

However it has been shown that the delivery of nuclease or antigens using integrative lentiviral particles leads to a toxicity for the resulting modified cells due to high and persistent expression of nucleases/proteins that should be transiently expressed. Combining a transient expression with a safe and efficient RNA delivery, the use of LentiFlash is preconized for gene editing applications (Knock-In and Knock-Out).

Lentiviral technology manufacturing from R&D to GMP grade

An attentive and expert customer support team

Vectalys is a real partner and brings expertise in project management, scientific design, project follow-up and quality of the results. The level of implication of Vectalys is dependent on our customer’s demand, from simply delivering a vector production to partnering on the whole project. Vectalys will also help you to define the best lentiviral vector batch to transduce your target cells with 100% efficiency.

Vectalys has very qualified and committed scientists, they deliver on what they promise. It's always a pleasure to work with them and their products.
Urs Lüthi, Associate Director and Deputy Head HTS at Idorsia Pharmaceuticals Ltd

Vectalys lentiviral batches have always been at the highest standard with excellent support from the scientific team: that has been a real asset for conducting our research projects over the years!
Thomas Moreau, Research Associate at University of Cambridge

"Vectalys has proposed effective solutions to all the problems encountered in the production of our viruses. They have been of great help in implementing our project."
Pierre, Team Leader

Vectalys team is a great support and technical expertise to answer about ours projects
Guillaume, preclinical researcher in neurosciences

Since we work with Vectalys, we have always had full satisfaction of Vectalys products
Yara Barreira, Unit director at INSERM

Thank you and all the staff at Vectalys for the great help at any level of the project.
Giuseppe, Head of Neuroscience, Italy

I would like to thank you again for your clear, efficient and successful work plan. It has been a very positive experience!
Janette, Scientist, UK

I thank you for the service Vectalys provides us and will for sure advertise it in the near future to our research network
Marc, Senior Scientist, Switzerland