Let’s say you have one or several great gene candidates, you are done with all the in vitro studies and you have been asked to validate your results in vivo. Looking at the bibliography, you inevitably fall into thousands of data showing wonderful results using the Adenoviral Associated Vectors (AAVs). Now you should be asking yourself “what serotype do I need to choose for my project?” and there you go back to bibliography trying to find out a clear answer to this question… and you won’t.
Delivery, delivery, delivery!
Dr Jennifer Doudna opened the last American Society of Gene and Cell Therapy Annual Meeting in Washington by highlighting the current challenges of gene editing :
• Controlling repair pathways
2 different letters and what else?
Everyone uses the word Transfection to designate the deliberate introduction of genetic material (DNA, RNA) into eukaryotic cells. The term is often used whatever the delivery tool but actually it should not!