Stem cells

Stem cells, transduction, high efficiency transduction, stable expression, serum free viral vectors, genetically modified cells, cell engineering, gene transfer, target validation, cell system development, in vitro systems.

Stem cells are not “permissive” preventing the use of conventional transfection techniques. The use of lentiviral vectors ensures high efficiency transduction and stable expression.

Vectalys has successfully transduced many stem cell types for a wide number of studies:

  • Mesenchymal stem cells immortalization during differentiation
  • Haematopoitic stem cells down regulation
  • Neural Stem Cells
  • Embryonic stem cells

Why Vectalys lentiviral vectors?

The viral vectors provided by Vectalys, by virtue of their high titre and purity, minimize the deleterious phenotypic changes that typically occur following transduction of target cells, such as loss of a sub-populations of transduced cells, and effects on proliferation, viability and differentiation of transduced cells.

Patented lentiviral production process

The Vectalys patented lentiviral production process which includes both serum free medium and a step of concentration and purification offers the following benefits for your experiment:

  • ensures between 75 - 100% transduction efficiency on stem cells
  • Vectalys lentiviral vectors enable to manage a dose effect on gene of interest (G.O.I.) expression by controlling the M.O.I.
  • Vectalys lentiviral vectors eliminate needs for antibiotic resistance selection steps

At Vectalys, we provide full teams of experts in cells engineering to support you and to fit the best your projects. We will help you to design the best experimental protocol for your gene delivery project.

Publications

  • Touboul T, Hannan, N, Corbineau S, Martinez A, Martinet C, Branchereau S, Mainot S, Strick-Marchand H, Pedersen R, DiSanto J, Weber A, Vallier L. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology, 2010, 51 (5), 1754-1765.
  • Lagresle-Peyrou C. et al. Human adenylate kinase 2 deficiency causes a profound haematopoietic defect associated with sensorineural deafness. Nat. Genet., 2009, 41 (1): 106–111.