Primary cells

Primary cells are not “permissive” preventing the use of conventional transfection techniques. The use of lentiviral vectors ensures high efficiency transduction and stable expression. Vectalys has successfully transduced many primary cell types for a wide number of studies:

• Endothelial cells
• Neurons
• Oligodendrocytes
• Neurospheres
• Pancreatic islets
• Hepatocytes
• Lymphocytes
• Dendritic cells
• Macrophages
• Corneal cells
• Monocytes
• . . .

Vectalys specific serum free purified vectors batches (C) for primary cells transduction show several advantages:

• The Vectalys patented lentiviral vectors do not affect the viability and the proliferation of your target cells.
• Vectalys lentiviral vectors enable to manage both % of positive cells and gene of interest (G.O.I.) level expression by controlling the M.O.I.
• Vectalys lentiviral vectors ensure that all the target cells are genetically modified – 100% transduction efficiency on primary cells and stem cells.
• Vectalys lentiviral vectors eliminate needs for antibiotic resistance selection steps

At Vectalys, we provide full teams of experts in cells engineering to support you and to fit the best your projects. We will help you to design the best experimental protocol for your gene delivery project by:

• Transducing target cells regarding the required gene level expression in two weeks
• Characterizing your genetically modified cells by RT-qPCR, western blot, immunochemistry or cytometry

Massart R, Guilloux JP, Mignon V, Sokoloff, Diaz J. Striatal GPR88 expression is confined to the whole projection neuron population and is regulated by dopaminergic and glutamatergic afferents. Eur. J. Neurosci., 2009, 30: 397–414.