Immortalized cell lines

The use of immortalized cell lines to study specific interactions in applications such as screening or protein production is well established. Stability of the cell line is crucial for consistent results. The use of lentiviral vectors ensures high transduction efficiency and a more stable expression when compared to conventional transfection techniques.

Since the creation of the company in 2005, we have run more than 500 generation cell lines projects, for many customer applications

• ADMET
• Protein production
• Reporter cell lines
• Tumoral cell lines

Vectors produced by Vectalys present 3 main advantages based on the absence of serum and on a reliable titer that:

• Ensures that the integration events occurs in every cells at a specific MOI– 100% transduction efficiency
• Enable management and control of the M.O.I. (dose effect)
• Eliminate needs for antibiotic resistance selection steps

At Vectalys, we provide full teams of experts in cells engineering to support you and to fit the best your projects. We will either take in charge your project or help you to design the best experimental protocol for your gene delivery project by:

• Transducing target cells regarding the required gene level expression in two weeks
• Ensuring monoclonal cell lines creation: analysis of 24 clones allow the selection of at least 3 clones that mimic the required specifications
• Characterizing your genetically modified cells by RT-qPCR, western blot, immunochemistry or cytometry

We will help you to design the best experimental protocol for your gene delivery project.