Lentiviral vectors

Vectalys platform is based on lentiviral based vectors that efficiently introduce human gene sequences into a wide variety of human cells to knock-in or knock-down specific proteins expression.

Since the creation of the company in 2005, we have generated more than 2000 viral vector batches, for many customer applications:

Gene transfer opens the way to develop cell and animal models by transferring target genes into functional and specific cells and tissues. Because primary cells are non permissive towards classical gene transfer technologies, viral based vectors derived from lentiviruses are the only efficient technology to achieve high expression of genes or silencing sequences in cells or tissues.

Lentiviral based vectors:

• allow the gene delivery into dividing and non-dividing cells.
• allow the gene delivery into primary cells and stem cells.
• are unique tools from in vitro transduction to in vivo injection.
• allow the stable expression of the sequence of interest (cDNA, shRNA, miRNA) due to the vector DNA integration into the host genomic DNA

Vectalys purified lentiviral vectors show several advantages:

• Vectalys lentiviral vectors do not affect the viability and the proliferation of your target cells.
• Vectalys concentrated and purified lentiviral vectors allow to reach 100% of cells transduced either on immortalized, primary or stem cells.
• Vectalys lentiviral vectors enable to manage a dose effect on the expression of the sequence of interest by controlling the M.O.I. (Multiplicity of Infection) due to a robust and standard vectors titration.
• Vectalys lentiviral vectors are time saving tools for stable cell line generation without any antibiotic selection.

Proprietary Vectalys vector production process

Vectalys has developed a platform of production, concentration and purification of viral vectors to provide the right vectors to the right application. We offer four levels of concentration and purification using different techniques based on ultrafiltration and chromatography to fit the best your project. The concentration and purification processes have an increasing quality in terms of contaminants removal as described on the following figure:

The most critical point in such concentration and purification strategies is the removal of serum contents. To overcome this issue, Vectalys has developed a process of production without serum, leading to a crude batch that exhibit a high transduction performance.

The removal of serum from the culture medium of the producer cells has a strong impact on the steps of ultrafiltration and chromatography since the initial concentration of protein and DNA contents are completely different in crude batches produced with or without serum.

The Vectalys lentiviral vectors purification processes prevent from any effect on cells toxicity, viability and proliferation after transduction.

Transducing your target eukaryotic cells with our serum free concentrated/purified vectors do not affect the viability of the cells, their capacity to proliferate in vitro, or their ability to progress down a pathway of differentiation (for example, when transducing stem cells). Such eukaryotic cells include, for example, immortalized cells, primary cells, stem cells or induced-pluripotent stem cells.