Lineage commitment for cell therapy
RUNX2 and DLX5 are master transcription factors inducing skeletogenesis in mesenchymal stem cells.
DLX5 has been implicated in the commitment of mesenchymal progenitors to the osteoblast lineage by the upregulation of RUNX2. RUNX2 is considered essential for osteoblastic differentiation.
Figure 4 shows lineage commitment in highly immature MSC-like cells toward an early osteoblastic lineage stage.
We first looked at the mRNA levels of transcription factors RUNX2 and DLX5 (induced by LentiFlash particles). At 58 h post-transduction, endogenous genomic RUNX2 or DLX5 mRNA levels significantly increased. In addition, the osteoblastic marker integrin-bone sialo-protein (iBSP) was upregulated in both conditions suggesting a commitment of MSC-l toward an early osteoblastic lineage stage.
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