Lentiviral based vectors deliver genes into a wide range of cell types stably with high efficiency. The cell types shown below were transduced with a lentiviral vector expressing a green fluorescent reporter gene and the transduction efficiency was analyzed by fluorescent activated cell sort analysis (FACS). Here, you can find several examples of cell transduction efficiencies on specific cells. For more details, you can contact: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Gene delivery in Immortalized cells (293T, HCT116, A549, B16, 4T1, HT19, CHO, HepG2)

All these cells are efficiently transduced with crude supernantant (10E6 TU/ml) at multiplicity of infection 5 to 10. At higher MOIs, the target gene expression level itself continues to increase in each transduced cell. For more details, you can contact: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Gene delivery in Primary cells

Human Blood cell transduction

Human naïve monocytes and dendritic cells isolated from peripheric blood have been successfully transduced with a lentiviral vector with a 90% success ratio.
A dose effect relationship can be obtained with dose incrementation as described below:

Human endothelial cell transduction (HUVEC)

Human endothelial cells have been isolated from umbilical blood (HUVEC). Transduction efficacy reachs 95% with a retrovirus or lentivirus pseudotyped with the VSVG.
Usually, transduction efficacy doesn’t exceed 40% with non viral agents.

Rat Cortical cell transduction (cns)

Rat Cortical cells have been obtained with pregnant rats.
The efficacy of transduction using lentiviral technology is close to 100%.

Gene delivery in stem cells

Human and murine hematopoietic stem cells (HSC)

Hematopoïetic stem cells have been obtained from Praxcell in Toulouse. Transduction can reach 60% in defined conditions that are provided with the vector batch.

Embryonic stem cells (ESC)

Genetic modification of embryonic stem cells is an outstanding tool for understanding development, gene function and influencing their biologic properties. At the present time, lentiviral vectors pseudotyped with the vesicular stomatitis virus G protein (VSV-G) have been most effective for stable gene transfer to embryonic stem cells.

Human Mesenchymal human cells (MSC)

Transduction efficiency reach 100% with a lentiviral derived vector pseudotyped by VSVG. On the following figure is described the increased transduction efficiency as the multiplicity of transduction raised.